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JPK CellHesion Module

Bruker JPK

CellHesion Module

Accessory for NanoWizard AFMs

The Bruker JPK CellHesion Module is an add-on for NanoWizard AFMs, for measuring cell adhesion and cell mechanics.

  • Long range force experiments with 100µm additional sample z-travel
  • Measure single molecules, entire cells, tissue, organelles and embryos
  • Simultaneous focus tracking (optional)
  • Integrates with advanced optical imaging eg DIC, phase contrast, fluorescence, confocal microscopy, super-resolution, FRET, FLIM, etc.

Contact us for more information and quotes:
+44 (0)1223 422 269 or info@blue-scientific.com

Contact Us CellHesion Module Brochure

JPK CellHesion Module

Applications

  • Cell adhesion
  • Cell mechanics
  • Biomaterials
  • Microbiology and virus research

Benefits

  • Enhance the capabilities of NanoWizard AFMs
  • Measure cell adhesion together with nanoscale imaging
  • Accurate, reproducible measurements
  • Integrates with advanced imaging techniques

Cell-Cell Adhesion

JPK CellHesion - How it Works

A single living cell is chemically bound to the cantilever sensor (eg with a fibronectin coating) under optical control.

CellHesion In a Microscope

The cell is brought into contact with the binding target on the substrate using a defined force. The binding target could be a molecular layer, implant surface, single cell or confluent monolayer. The substrate can be a slide, coverslip or Petri dish.

Measure cellular interaction

After a specified length of time, the cantilever is retracted vertically (on the z-axis) using a piezo actuator. This separates the cell from the substrate cell.

Cellular interaction analysis

If there is adhesion, the cell resists the effort to remove it. This bends the cantilever, which is measured by a detector.

In physical terms, the cantilever is a leaf spring, so the adhesive force and energy can be calculated by measuring the bending.

This enables you to identify single-molecule binding events that contribute to the adhesion. By repeating the test with various cells, targets and conditions you can collect statistically relevant information.

Cell-Substrate Adhesion

The force vs. distance curve, enables you to calculate:

  • Single molecule events
  • “Work of removal”
  • Tether formation
  • Maximum adhesion force
  • Viscoelastic parameters

Cell-Substrate Adhesion

Click to enlarge

Example Applications

  • Stiffness and elasticity mapping
  • Single cells to substrates and tissues
  • Cell-cell and cell-substrate interaction
  • Cell adhesion and tether formation
  • Biomaterial studies, biofouling, biosensors, capsules
  • Implant coatings and cellular biochips
  • Microbiology and virus research
  • Pharmaceuticals eg drug delivery mechanisms
  • Food, paper and textiles eg fibres, coatings and powders in air or liquid
  • Binding studies eg receptor/ligand and antibody/antigene
  • Testing functionalised surfaces

Cell Adhesion Measurement

Measuring the adhesion of cell-cell interaction between MDCK cells (click to enlarge)

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